Curriculum Vita: Andrew J. Howard

Andrew J. Howard, Ph.D.

EDUCATION

1975, B.A., Biophysics, Pomona College, Claremont, CA.
1981, Ph.D., Physics Dept., University of California, San Diego.;
   Dissertation Advisor: Xuong Nguyen-huu.
   Project: software development for crystallographic data processing.
1981-1983, Postdoctoral Research, UC Los Angeles .
   Chemistry Dept. and Molecular Biology Institute.    Advisor: David S. Eisenberg.
   Research specializations: structure of RuBisCO; crystallographic methods development.
1983-1984, Postdoctoral Research, Inhalation Toxicology Research Institute,
   Albuquerque, NM. Advisor: Charles Mitchell.   Research specialization: energy-related toxicology

EMPLOYMENT

1984-1993,Genex Corporation and Enzon, Inc.,
   Gaithersburg, MD; Corporate Research Scientist.
   Wrote XENGEN software for processing Siemens Area Detector data.
   Solved or worked on structures of numerous proteins.
   Executed Phase I NSF SBIR for crystallographic software development.
1993-1995, Molecular Simulations, Inc., Burlington, MA/ Rockville, MD
   Senior Scientist, Life Sciences.
    Directed NSF-, NIH- and NIST-funded software development projects
   Participated in crystallographic research at
     CARB, Rockville, MD, as a visiting scientist.
1995-     Associate Professor, Biology Division, BCPS Dept.,
Illinois Institute of Technology, (tenured 2000)
   Research specializations:
crystallographic methods development; structural genomics; pharmaceutical crystallography
1995-     Associate Director, Director, and Chief Scientific Officer,
Industrial Macromolecular Crystallography Association CAT,
   Advanced Photon Source, Argonne National Laboratory

TEACHING EXPERIENCE

1973-79: Teaching assistant, physics, Pomona Collega and UCSD
1981: Taught organic and biological chemistry at UCLA (175 students)
1981-1995: Instructed users of XENGEN software worldwide.
1995-1999: Faculty member, Cold Spring Harbor Laboratory Crystallography School
1997- Freshman survey courses in scientific practice, IIT.
1998- Human biology and biochemistry courses, Biology Division, BCPS Department, IIT
2000- Managed and found speakers for biology colloquium class
1998- Radiation biophysics as part of IIT's Health Physics graduate program
Graduate student supervision:
Two completed Ph.D.'s, four in progress; three master's completed.
Undergraduate research supervision:
Nine undergraduate students have worked at least one semester each.

RESEARCH SUPPORT

1990-1995: Four SBIR projects; sole author of two. Total funding around $1M.
1996- Co-PI or PI on IMCA-CAT project
to develop pharmaceutical crystallographic facilities at the APS.
Total funding around $11M.
1996-2002: PI on an NIST specially-funded project. $300K.
1998- Co-PI on NIH P01 in Structural Genomics.
Total funding around $10M.
2004- , Principal investigator, NIH R01;
Proposal under development involving crystallographic software; $700K.

SCIENTIFIC COMMUNITY SERVICE

1991- NIH, NSF, NASA peer-review panels.
1998-2000: Co-editor, mmCIF software standards
1997-1999 Founder and first Chair, CAT Director's Council, APS
2000 Chair, Diffraction Methods in Molecular Biology Gordon Conference

SELECTED PUBLICATIONS (out of 41)

Weber, P.C. , Howard, A., Xuong, Ng -h and Salemme, F.R. (1981) Crystallographic structure of Rhodospirillum molischianum ferricytrochrome c' at 2.5 Å resolution. J. Mol. Biol. 153: 399-424.
Hamlin, R.C., Cork, C. Howard, A. Nielsen, C., Vernon, W., Matthews, D., and Xuong, Ng.-h. (1981) Characteristics of a flat multiwire area detector for protein crystallography, J. Appl. Crystallogr. 14: 85-93.
Howard, A.J. (1982) Computing requirements for area detector data collection, in : Computational Crystallography (Sayre, D., ed.: Oxford, Clarendon Press), pp. 29-40.
Howard, A.J. Nielsen, C.P., Xuong, Ng.-h (1984) Software for a multiwire area detector. Methods in Enzymology 114: 452-472.
Howard, A.J. Mitchell, C.E., Dutcher, J.S., Henderson, T.R. and McClellan, R.O. (1986) Binding of nitropyrenes and benzo[a]pyrene to mouse lung deoxyribonucleic acid after pretreatment with inducing agents. Biochemical Pharmacology 35: 2129-2134.
Howard, A.J. Gilliland, G.L., Finzel, B.C. Poulos, T.L. Ohlendorf, D.H. and Salemme, F.R. (1987) Use of an imaging proportional counter in macromolecular crystallography. J. Applied Crystallogr. 20: 383:387.
Poulos, T.L. and Howard, A.J. (1987) Crystal structures of metyrapone- and phenyimidazole-inhibited complexes of cytochrome P-450cam. Biochemistry 26: 8165-8174.
Howard, A.J. and Poulos, T.L., (1992) Methods in macromolecular crystallography. chapter in Advances in Biophysical Chemistry 2: 1-36.
Howard, A.J. (2000) Data processing in macromolecular crystallography. Chapter in: Crystallographic Computing 7: Proceedings from the Macromolecular Crystallographic Computing School, 1996. P.E. Bourne and K.D. Watenpaugh, eds. Oxford: Oxford University Press.
Thoden, J.B., Miran, S.G., Phillips, J.C., Howard, A.J., Raushel, F.M., Holden, H.M. (1998) Carbamoyl phosphate synthetase: Caught in the act of glutamine hydrolysis. Biochemistry 37: 8825-8831.
Howard, A. (1998) Participation of pharmaceutical companies in synchrotron radiation research. Nature Structural Biology 5: 623-626.
Howard, A. (1999) Macromolecular crystallography at third-generation synchrotron sources. Chapter for Third Generation Hard X-ray Synchrotron Radiation Sources: Source Properties, Optics, and Experimental Techniques, Dennis Mills, ed. Wiley: 2002.
Eisenstein, E., Gilliland, G.L., Herzberg, O., Moult, J., Orban, J., Poljak, R.J., Banerjei, L., Richardson, D., and Howard, A.J. (2000) Biological function made crystal clear - annotation of hypothetical proteins via structural genomics. Current Opinions in Biotechnology 11: 25-30.
Ladner, J.E., Reddy, P., Davis, A., Tordova, M., Howard, A.J., and Gilliland, G.L. (2000) The 1.30 Å resolution structure of the Bacillus subtilis chorimate mutase catalytic homotrimer. Acta Crystallographica D 56: 673-683.
Gallagher, D.T., Mayhew, M., Holden, M.J., Howard, A., Kim, K.J., and Vilker, V.L. (2001) The crystal structure of chorimate lyase shows a new fold and a tightly retained product. Proteins 44: 304-311.
Lim, K., Zhang, H., Tempczyk, A., Bonander, N., Toedt, J., Howard, A., Eisenstein, E., and Herzberg, O. (2001) Crystal structure of YecO from Haemophilus influenzae (HI0319) reveals a methyltransferase fold and a bound S-Adenosylhomocysteine. Proteins 45: 397-407.
Sanders, D.A.R., Moothoo, D.N., Raftery, J., Howard, A.J., Helliwell, J.R., and Naismith, J.H. (2001) The 1.2 Å resolution structure of the con A-dimannose complex. J. Mol. Biol. 310: 875-884.
Lim, K., Zhang, H., Tempczyk, A., Krajewski, W., Bonander, N., Toedt, J., Howard, A., Eisenstein, E., and Herzberg, O. (2003) Structure of the YibK methyltransferase from Haemophilus influenzae (HI0766): a cofactor bound at a site formed by a knot. Proteins: Structure, Function, and Genetics 51:56-67.
Howard, A.J. (2002) The X-GEN software package for crystallographic data processing. Journal of Applied Crystallography, in preparation.