Syllabus for Biology 533, Spring 2012: Structural Biology Unit

Week Lecture Activity Writeup
1 1. Safety lecture
2. X-ray scattering and diffraction
1. Demonstration (by TA) of crystallization setup
2. Prepare buffers and protein samples
Submit GUP and ESAF applications
2 Theory of macromolecular crystallization Set up crystal trays (recipes and screens) Describe crystallization setup
(4 paragraphs and table)
3 Theory of fiber crystallography Prepare fiber samples Describe fiber sample setup
(4 paragraphs and table)
4 Fiber and single-crystal diffraction Fiber diffraction data processing Describe fiber data processing
(5 paragraphs incl. table)
5 Onsite Data Collection at the APS Describe experiments (2 tables and 3 paragraphs)