Andrew J. Howard, Ph.D.
office:Center for Synchrotron Radiation Research and Instrumentation
- Department of Biological, Chemical, and Physical Sciences
- Illinois Institute of Technology
3101 South Dearborn St. Chicago IL 60616
- 312-567-5881; fax 312-567-3576 howard@iit.edu
EDUCATION
- 1975, B.A., Biophysics,
Pomona College, Claremont, CA.
- 1981, Ph.D.,
Physics Dept., University of California, San Diego.
- Dissertation Advisor: Xuong Nguyen-huu.
-
1981-1983, Postdoctoral Research, UC Los Angeles .
- Chemistry Dept. and Molecular Biology Institute.
Advisor: David S. Eisenberg.
- 1983-1984, Postdoctoral Research,
Inhalation Toxicology Research Institute,
- Albuquerque, NM. Advisor: Charles Mitchell.
EMPLOYMENT
- 1984-1993,Genex Corporation and Enzon, Inc.,
Gaithersburg, MD, Corporate Research Scientist.
- 1993-1995, Molecular Simulations, Inc.,
Burlington, MA/ Rockville, MD
- Senior Scientist, Life Sciences.
1995-present;Advanced Photon Source, Argonne National Laboratory
Associate Director (95-96), Director (96-02), and Chief Scientific Officer
(02-present), Industrial Macromolecular Crystallography Association
Collaborative Access Team.
- 1995-present, Illinois Institute
of Technology, Chicago, IL.
- Research Associate Professor (95-97), Associate Professor (97-present),
Department of Biological, Chemical, and Physical Sciences.
TEACHING EXPERIENCE
1973-79 Teaching assistant in freshman physics at Pomona
College and UC San Diego
1981 Taught organic and biological
chemistry at UC Los Angeles (175 students)
1987-93 Instructed users of XENGEN software worldwide, as
needed
1996- , Created and teach Introduction to the Professions
at IIT;
this is a survey course taken by all freshman biology, chemistry, and physics
majors
1998- Research advisor to three full-time graduate students.
1998- Recreated and taught graduate course in radiation
biophysics at IIT
1999 Taught freshman human biology to 61 students at IIT
1999- In charge of biology colloquium series at IIT.
2002- Built and taught junior-level undergraduate biochemistry,
chiefly to premedical students and molecular biology majors.
RESEARCH SUPPORT: Past
1991-1995 Principal Investigator, National Science Foundation
Small Business Innovative Research Grant.
1997-99 Co-principal investigator, IITRI Institutional Research
and Development Project,
with K.Stagliano at IIT.
1998-2001, Principal Investigator, NIST contract,
"Structural Studies of Enzymes Involved in Aromatic Amino Acid
Biosynthesis". IIT budget: approx. $60,000 per year.
RESEARCH SUPPORT: Current
1998- , Principal Investigator (1998-2002), Co-Principal
Investigator (2002-present), contract between IIT
and the Industrial Macromolecular Crystallography Association (IMCA).
Contract specifies IIT as developer and manager of experimental facilities
for IMCA. Operating budget: approximately $1,000,000 per year. Capital budget:
$250,000 - $900,000 per year depending on requirements.
1998- , Principal Investigator, NIH subcontract,
"X-ray diffraction measurements at the Argonne Synchrotron".
Part of multi-institution program project grant from the National Institutes
of Health to the Maryland Biotechnology Institute, The Institute for Genomic
Research, and IIT. IIT budget: $250,833 for five years. Program project
title: "From Genomic Sequences to Protein Structure and Function".
SCIENTIFIC COMMUNITY SERVICE
1991- , Member, NIH Special Study Section 6.
1992-1993 NSF Small Business Innovation Research Grant proposal
review; two sessions.
1997-1999 Founder and first Chair, CAT Director's Council
at the Advanced Photon Source
1998-2000, Vice chair (1998) and chair (2000),
Diffraction Methods in Molecular Biology Gordon Conference;
Proctor Academy, Andover, NH
1998-2001, NIH ad hoc site-visit panels to review Research
Resource grants.
SELECTED PUBLICATIONS
Weber, P.C., Bartsch, R.G., Cusanovich, M.A., Hamlin, R.C., Howard, A.,
Jordan, S.R., Kamen, M.D., Meyer, T.E., Weatherford, D.W., Xuong, Ng.-h.,
and Salemme, F.R. (1980) Structure of cyctochrome c': A dimeric, high-spin
haem protein. Nature 286:302-304.
Howard, A.J. (1982) Computing
requirements for area detector data collection, in : Computational Crystallography
(Sayre, D., ed.: Oxford, Clarendon Press), pp. 29-40.
Howard, A.J. Nielsen, C.P.,
Xuong, Ng.-h (1984) Software for a multiwire area detector. Methods in
Enzymology114: 452-472.
Howard, A.J. Mitchell, C.E.,
Dutcher, J.S., Henderson, T.R. and McClellan, R.O. (1986) Binding of nitropyrenes
and benzo[a]pyrene to mouse lung deoxyribonucleic acid after pretreatment
with inducing agents. Biochemical Pharmacology35: 2129-2134.
Howard, A.J. Gilliland, G.L.,
Finzel, B.C. Poulos, T.L. Ohlendorf, D.H. and Salemme, F.R. (1987) Use of
an imaging proportional counter in macromolecular crystallography. J. Applied
Crystallogr.20: 383:387.
Poulos, T.L. and Howard, A.J.
(1987) Crystal structures of metyrapone- and phenyimidazole-inhibited complexes
of cytochrome P-450cam. Biochemistry26: 8165-8174.
Howard, A.J. and Poulos, T.L.,
(1992) Methods in macromolecular crystallography. chapter in Advances in
Biophysical Chemistry2: 1-36.
Howard, A.J. (2000) Data processing
in macromolecular crystallography. Chapter in: Crystallographic Computing
7: Proceedings from the Macromolecular Crystallographic Computing School,
1996. P.E. Bourne and K.D. Watenpaugh, eds. Oxford: Oxford University Press.
Thoden, J.B., Miran, S.G.,
Phillips, J.C., Howard, A.J., Raushel, F.M., Holden, H.M. (1998) Carbamoyl
phosphate synthetase: Caught in the Act of Glutamine hydrolysis. Biochemistry
37: 8825-8831.
Howard, A. (1998) Participation
of pharmaceutical companies in synchrotron radiation research. Nature Structural
Biology5: 623-626.
Howard, A. (2002) Macromolecular
crystallography at third-generation synchrotron sources. Chapter for Third
Generation Hard X-ray Synchrotron Radiation Sources: Source Properties,
Optics, and Experimental Techniques. D.M. Mills, ed.
Wiley: 406pp.
Eisenstein, E., Gilliland,
G.L., Herzberg, O., Moult, J., Orban, J., Poljak, R.J., Banerjei, L., Richardson,
D., and Howard, A.J. (2000) Biological function made crystal clear - annotation
of hypothetical proteins via structural genomics. Current Opinions in Biotechnology
11: 25-30.
Lim, K., Zhang, H., Tempczyk, A., Krajewski, W., Bonander, N.,
Toedt, J., Howard, A., Eisenstein, E., and Herzberg, O. (2003)
Structure of the YibK methyltransferase from Haemophilus influenzae
(HI0766): a cofactor bound at a site formed by a knot. Proteins:
Structure, Function, and Genetics 51:56-67.
Howard, A.J. (2004) The X-GEN
software package for crystallographic data processing. Journal of Applied
Crystallography, in preparation.